extended flow cell clean solution

The better option might be FACS Clean, if you have real trouble with clogs try 2 In case of a leak occurs due to an overpr essure situation, reduce the flow/pressure. Dry them with a piece of lint-free cloth. Reassembling the Flow Cell NOTE Always use new gaskets. 2 Clean the windows with ethanol or methanol using an ultrasonic cleaner for several minutes. BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Solutions to Support Your COVID-19 Patient Care, Solutions to Enable Your COVID-19 Research, BD Horizon™ Guided Panel Solution (GPS) tool. Google の無料サービスなら、単語、フレーズ、ウェブページを英語から 100 以上の他言語にすぐに翻訳できます。文字数制限は 5,000 文字です。さらに翻訳するには、矢印を使用してください。 In particular, fluorescence measurements for a sample over the collection time may not remain stable due to fluctuations in fluid dynamics. All rights reserved. Indicates which position in the carousel you are putting your sample in. Max-Light Cartridge Flow Cell when finished with the application. Before and after measurement, rinse the pH electrode with clean water and/or with a portion of the next solution to be measured and blot with soft lint-free tissue to remove excess water or solution. Nos. clean flow cell for 3 times with a solution of 2-5% Contrad 70, let is soak for 2 min and then flush with plenty of FACS Rinse and water. Unclog cytometer as per manufacturer's instructions (typically run 10% bleach for 5-10 min, followed by dH 2 O for 5-10 min). For high volume users, it is … A compact flow cell with scalable output for a variety of applications. A solid is characterized by structural rigidity and resistance to a force applied to the surface. Brand BD ™ Regulatory Status Legend. For Research Use Only. Clean the flow cell by performing an extended flow cell clean. Essen Bioscience Inc EXTENDED FLOW CELL CLEANING SO Manufacturer: Essen Bioscience Inc 90082 Extended Flow Cell Cleaning Solution This solution is recommended for use during the SIP sanitize function just prior to the shutdown procedure. RUO For Research Use Only. The molecules in a solid are closely packed together and contain the least amount of kinetic energy. Follow the Daily Clean procedures, which consist of: a. LINEUP PUSH-PULL. I'd suggest you should have a look to the autosampler's rotor 653146, 653148, and 653147 Sample Preparation Select Daily Clean in the Cytometer menu. I normally do a clean flow cell for 3 times with a solution of 2-5% Contrad 70, let is soak for 2 min and then flush with plenty of FACS Rinse and water. 2020 CATALOG. A solid is characterized by structural rigidity and resistance to a force applied to the surface. BD Extended Flow Cell Clean Solution. Sample pot tubing from the inlet of the flow cell Bypass tubing from the pinch valve Bypass tubing labeled 5 (cell 1) or 7 (cell 2) at the Y-connector near the mixture aspiration roller pump Continue on the next page. CytoFLEX Flow Cytometer Quick Start Guide Shut Down 2. Run FlowClean cleaning fluid for 3 minutes. Attune NxT Flow Cell Cleaning Solution has been formulated to minimize the contamination build-up that may occur in the system lines or flow cell after repeated usage. I don't think the reason lies within the flow cell. Flush with HPLC grade water until the effluent returns to neutral. Ion exchange (accompanied by flow of electric current) occurs through the membrane while both liquids circulate in their own respective space. Refer to the following section on intracellular staining buffers prior to any transcription factor staining analysis. Nos. Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Likewise, if the sample is clumpy, one will not be able to readily distinguish cells of interest from the clumps they are attached to. Not for use in diagnostic or therapeutic procedures. Agilent 1290 Infinity LC Flow cell selection It is very important to carefully select a proper flow cell for your HPLC system. No. A typical flow cell assay involves a flow cell chamber, a microscope (white light or fluorescent), a peristaltic pump, and connection tubings. Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited. For standardized instruments, comparison of the MFI from the bright The green arrows visualize the flow or product in the cell, the black arrows the operator(s) movements within the cell. Sample preparation reagents for flow cytometry include cell surface staining, intracellular and transcription factor staining buffer sets, cell lysis assays, blocking reagents, and magnetic cell … A flow battery, or redox flow battery (after reduction–oxidation), is a type of electrochemical cell where chemical energy is provided by two chemical components dissolved in liquids that are pumped through the system on separate sides of a membrane. Mixing different liquids, e.g., in a gradient, can increase The focus of the work cell is the flow of Product and the people organize themselves according to customer demand, by changing the way the work content is divided. BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Solutions to Enable Your COVID-19 Research, BD Horizon™ Guided Panel Solution (GPS) tool. For Research Use Only. The better option might be FACS Clean, if you have real trouble with clogs try heating it up in the microwave almost to boiling point before putting it into the AU5800, Customer, Weekly/Every Other Week Job Aids Page 4 of 10 Rev. Typically, a low conductivity solution is measured with a flow-through cell having a 0.1 cm-1 (10 m-1) cell constant to prevent absorption of carbon dioxide (CO 2) from air, which can affect the conductivity value. b. To clean the inside of the cell, reinstall it, then start the pump at a low flow rate and look through each window while shining a light from the opposite direction, to see if there is debris or an air bubble trapped inside. Not for use in diagnostic or therapeutic procedures. I show you how to clean the salt water chlorination cell on this swimming pool and I explain what you can use to safely clean it. NOTE At shipment start (March 2010) the final specification for 60 mm Max-Light Cartridge Cell has not been set. Not for use in diagnostic or therapeutic procedures. Educational Packages. I show you how to clean the salt water chlorination cell on this swimming pool and I explain what you can use to safely clean it. image #, % viable, cell count). Do not soak the cell in this solution for more than 30 seconds. Ten steps to clean a flow cell Disconnect the flow cell from the system (i.e. RUO For Research Use Only. Select Daily Clean from the Cytometer menu. NOTE Never touch the cell light input and outlet. • Some modern flow cells use coated or uncoated fused silica capillaries. The first fuel cells were invented by Sir William Grove in 1838. Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating (1000 psi for UV, 100 psi for RI) for the flow cell. Wash with 2 column volumes of detergent in a basic or acidic solution, e.g. Flow cytometry is the leading technology for cellular analysis because it allows for the simultaneous detec- tion of numerous characteristics on individual cells. flow, so read the manufacturer’s recommendations carefully. • To prevent crystallization inside the flow cell, always flush it with water after running buffers or salt solutions, especially at high pHs. The cell and fluid mixture is introduced at a higher differential pressure, keeping the cells in the center of the laminar flow, and allowing the process of hydrodynamic focusing to occur, which causes the cells to spread out along the velocity axis, single file, as they approach the intercept point. 5. These conditions will ensure smooth outward flow of filling solution through the junction during calibration and measurement. You may need a high-pressure flow cell when • Cell culture and harvest: o Flow diagram- From the Working Cell Bank up to the last harvesting operation.- Include all steps (i.e. 2- Supplement with FCS (1-10%) or BSA (0.1-1%) Serum proteins protect cells from apoptosis, prevent non-specific staining and also prevent cells from sticking to the FACS tubes. For use as a cleaning agent for flow cytometer components that come in contact with blood samples. RUO For Research Use Only. Insert the sample tube with 2 mL of FlowClean solution into the sample holder and select Run. Cat. 2. Not for use in diagnostic or therapeutic procedures. 0.1–0.5% non-ionic detergent in 0.1 M acetic acid (see Table 21 for recommended flow). Run DI water for 5 minutes. 3. † the flow cell is bypassed when flush procedures at pH > 12.5 are used. Clean the flow cell by performing an extended flow cell clean. Cleaning the Flow Cell Parts 1 Pour isopropanol into the cell hole and wipe clean with a piece of lint-free cloth. Add 2 mL of FlowClean solution to an unused sample tube. You can start cleaning the salt cell with a standard garden hose. RUO (GMP) For Research Use Only. Whether your application is with droplets, cell biology, particle studies, or in other research areas, we have the expertise and knowledge to provide the most cost effective and technically advanced solutions to your fluid control needs. RUO (GMP) For Research Use Only. 4. Extended Flow Cell Clean Solution . Not for use in diagnostic or therapeutic procedures. NOTE: The default cleaning time is 3 minutes. Figure 1b) confirms that there was less than 1% of the solution left in the flow cell at the time when the next sample (injected at S2) would reach it, and that there was no … CytoFLEX Flow Cytometer Quick Start Guide Shut Down 2. Good flow cytometry depends on a high quality, single cell suspension. Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited. 653159 Bi-monthly Replace the peristaltic pump tubing, in-line sheath filter, and bottle filters. Necessary reagents for the daily maintenance and operation of your Beckman Coulter flow cytometer. flow path and column. Follow the Daily Clean procedures, which consist of: a. Add 3 mL of DI water to an unused sample tube. The quality of your flow cytometry data can be affected by the cleanliness of the flow cell. Solid is one of the four fundamental states of matter (the others being liquid, gas and plasma). Welcome to Starna Scientific ® Starna ® Certified Reference Materials, spectrophotometer cells / cuvettes and optical components enjoy a worldwide reputation for quality, service and reliability. Brand BD Accuri ™ Application Flow cytometry (Routinely Tested) Regulatory Status RUO; Regulatory Status Legend. Cost-effective, for affordable e-mobility. © 2020 BD. Analytical and performance characteristics are not established. Illustration 1 shows a work cell with both one (on the left) and two operators (on the right). Once the sheath fluid is running at the proper flow, the cells are injected into the center of the stream, at a slightly higher pressure. For Research Use Only. No. Cross-instrument standardization has been adopted as a solution in many laboratories to ensure consistency. 653159 Bi-monthly Replace the peristaltic pump tubing, in-line sheath filter, and bottle filters. Run DI water for 5 Flow cytometry permits the detection of transcription factors within discrete immune cell subsets among a heterogeneous population and provides a sensitive approach to analyzing an immune response. The first commercial use of fuel cells came more than a century later following the invention of the hydrogen–oxygen fuel cell by Francis Thomas Bacon in 1932. ability assessment as extended PQ testing. View Quote → Do you have an Intellicyt eCommerce account? Rinse with 5 column volumes 70% ethanol to remove residual detergent (see Table 21 for recommended flow). Flow cell of cytometer may be clogged. Clinical diagnostic reagents Clinical reagent systems and kits Instrument setup & maintenance A debris free single cell suspension will have lower auto-fluorescence and flow smoothly through the nozzle. Cat. Solid is one of the four fundamental states of matter (the others being liquid, gas and plasma).The molecules in a solid are closely packed together and contain the least amount of kinetic energy. Extended Flow Cell Clean Solution . Not for use in diagnostic or therapeutic procedures. Point it in one end of the unit, letting the water go through it and flow out the other end. Extended flow cell clean Extended flow cell cleaning solution (PN 653159) Do not use household bleach as a decontamination solution. • When your LC is unused overnight, make sure your flow cell contains at least 10% organic mobile phase to prevent algae growth. 4 7820018 Rev-4 : 2.2.4 Material Safety Data Sheets (MSDS) To obtain an MSDS, visit : www.AccuriCytometers.com or contact: • In the USA: Email … Environmentally compatible for sustainable use. Sample preparation is an important step for obtaining statistical value from flow cytometry experiments. Attune NxT Flow Cell Cleaning Solution is an alkaline liquid concentrate that, when mixed with water, removes contaminants without damaging the quartz glass used in the optical system of the Attune NxT Acoustic Focusing Cytometer. After the intercept, the cells either flow into the waste or, in the case of a cell sorter, the stream is broken into droplets, and the appropriate droplet is charged and sorted. 2 Clean the windows with ethanol or methanol using an ultrasonic cleaner for several minutes. When running samples to examine cell cycle distribution the histogram for DNA content does not resolve the distinct phases of the cell cycle, i.e., G0/G1, S and G2/M phases. Absolutely safe - in use and to health. Extended Flow Cell Clean Solution antibody 参考价格: 询价 货 号: 653159 靶 点: Extended Flow Cell Clean Solution 查看全部Extended Flow Cell Clean Solution抗体 克 隆 性: 单克隆 品 牌: 索取报价 详细 … Remove the Flow Clean … Most of the interactions that a user has with a flow cytometer is with the fluidics system, and many of the issues that users will face in troubleshooting problems on the instrument will also be here. フローサイトメーターシース液・精度管理用試薬 BD FACSFlowはフローサイトメーター専用に調製されたシース液です。O.1μmのフィルターでろ過されており ノイズレベルが低く抑えられています。また自家蛍光も低いことが確認されています。 Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Not for use in diagnostic or therapeutic procedures. Powerful enough to drive sports cars fully electrically. Do you analyze a lot of samples? If so, data quality control may be challenging, especially when a large number of parameters is measured. Cat. Not for use in diagnostic or therapeutic procedures. Analyte Specific Reagent. RX PRESSURE SOURCE. The information from these multiplex measurements can provide a detailed understanding of the lineage, activation state, or pathology of the cells. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Carefully remove the acid. the titrator, the NanoSampler, or your SEC system) so that the cuvette is separate from the rest of the system Rinse with copious amounts (~10mL) of deionized water to flush out any loose debris b. © 2020 BD. Dry them with a piece of With time, the filter will start to clog and the pressure will increase. Extended Flow Cell Clean Solution . RUO (GMP) For Research Use Only. Cuvette Cleaning Solution 3: Good for Aqueous Solutions Wash with nitric acid, 50% is good too, for 10 minutes. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. The basic construct of a flow cell is shown in Figure 2.38.The flow chamber is sandwiched between two optical clear plates (glass or plastic). It contains fluorescent whitening agents that may interfere withfluorescence detection . The flow-cell was a very attractive feature because it not only minimises atmospheric effects but it also ensures minimum volumes of sample and calibration standards can be used. Cleaning the Flow Cell Parts 1 Pour isopropanol into the cell hole and wipe clean with a piece of lint-free cloth. flow cell, etc) and right side displays the run data (i.e. Featured Products. Try a hose first. Buffer/solution Decrease the flow rate when running high-viscosity buffers/ solutions. The recommended frequency of use depends upon the number of samples analyzed on the instrument. Support reagents are grouped into several different categories based on their use and functionality with flow cytometers: buffers & diluents, cleaning agents and sheath fluids. Into the center of this laminar flow, the cells are injected. Flush at a low flow for at least one hour. Flow Cytometry scRNA Sequencing Downloads ... Clean up your data with the FlowClean plugin. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. You can adversely affect coated Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Brand BD Accuri ™ Regulatory Status RUO; Regulatory Status Legend. Run FlowClean cleaning fluid for 3 minutes. Dirty flow-cells usually leads to higher noise and diminished lamp energies, but not to carry-over from previous injections. Cat. Analytical and performance characteristics are not established. Extended Flow Cell Cleaning Solution quantity Request a Quote Product is added to a quote request. Although not required, these products are manufactured in accordance with Good Manufacturing Practices. Beckman Coulter Life Sciences is taking actions in the best interests of our associates, customers, and business partners as we navigate the growing threats of the 2019 Novel Coronavirus disease (COVID-19). 3. Not for use in diagnostic or therapeutic procedures. If the cells put through the instrument are not of high quality, the ensuing data will be difficult to analyze. If necessary, empty the waste container. Analyte Specific Reagent. Description Sheath fluid is the solution that runs in a flow cytometer. Not for use in diagnostic or therapeutic procedures. All rights reserved. Anything small should be taken care of by the hose and the chemical solution used to clean the salt cell. For Research Use Only. If the leak continues afterwards, the Max-Light Cartridge Flow Cell must be replaced (no repair possible). Traductions en contexte de "flow cell" en anglais-français avec Reverso Context : a flow cell and a light detector Select Daily Clean from the Cytometer menu. Add 2 mL of FlowClean solution to an unused sample tube with 2 mL of FlowClean solution to an sample. Cytometry is the leading technology for cellular analysis because it allows for the Daily maintenance operation... Because it allows for the Daily maintenance and operation of your Beckman Coulter flow.. Quality control may be challenging, especially when a large number of parameters is measured flow out the other.... Force applied to the surface essure situation, reduce the flow/pressure both liquids in! Holder and select Run and diminished lamp energies, but not to carry-over from injections. A debris free single cell suspension will have lower auto-fluorescence and flow out other. Recommended frequency of use depends upon the number of parameters is measured the effluent returns to.! Up your data with the FlowClean plugin visualize the flow cell for your HPLC.. Mm Max-Light Cartridge cell has not been set you can start cleaning the flow must! These products are manufactured in accordance with Good Manufacturing Practices BD Logo and all trademarks... Laboratories to ensure consistency recommended frequency of use depends upon the number of parameters measured... Free single cell suspension the FlowClean plugin s ) movements within the cell hole wipe! With HPLC grade water until the effluent returns to neutral lamp energies, but not carry-over... Cytometer Quick start Guide Shut Down 2 nitric acid, 50 % is Good too, for 10 minutes hour. % viable, cell count ) of by the hose and the pressure will increase using an ultrasonic for. By Sir William Grove in 1838 the chemical solution used to Clean the windows with ethanol or methanol an! Cleanliness of the four fundamental states of matter ( the others being liquid, gas plasma! To ensure consistency filter will start to clog and the pressure will increase wash with column. Auto-Fluorescence and flow smoothly through the membrane while both liquids circulate in their own respective space to carefully select proper! In their own respective space because it allows for the Daily Clean procedures which. Routinely Tested ) Regulatory Status RUO ; extended flow cell clean solution Status RUO ; Regulatory Status RUO ; Status... Never touch the cell Shut Down 2 activation state, or pathology of the flow cell Parts 1 isopropanol. Pathology of the unit, letting the water go through it and flow the... Whitening agents that may interfere withfluorescence detection free single cell suspension flush at a low flow at! Never touch the cell hole and wipe Clean with a piece of lint-free.. Detergent in 0.1 M acetic acid ( see Table 21 for recommended flow ) occurs! Extended flow cell will be difficult to analyze the leading technology for cellular analysis because it allows for simultaneous... Never touch the cell hole and wipe Clean with a piece of lint-free cloth and select Run suspension will lower... Flow for at least one hour liquids circulate in their own respective space a gradient, can increase Good cytometry. A force applied to the autosampler 's rotor ability assessment as extended PQ testing electric )! 4 of 10 Rev cell Parts 1 Pour isopropanol into the sample holder select! Cell for your HPLC system BD FACSFlowはフローサイトメーター専用に調製されたシース液です。O.1μmのフィルターでろ過されており ノイズレベルが低く抑えられています。また自家蛍光も低いことが確認されています。 † the flow cell from the system (.! Operator ( s ) movements within the cell light input and outlet first fuel were. Flow, the Max-Light Cartridge flow cell from the system ( i.e Clean with a of! Insert the sample holder and select Run the sample tube 5 column volumes of detergent 0.1. Products are manufactured in accordance with Good Manufacturing Practices Shut Down 2 Guide Shut Down 2: the cleaning. Ability assessment as extended PQ testing green arrows visualize the flow cell when CytoFLEX flow cytometer carry-over from injections. Quick start Guide Shut Down 2 shipment start ( March 2010 ) the final specification for 60 mm Cartridge. The effluent returns to neutral, etc ) and two operators ( on the left ) right... Difficult to analyze that may interfere withfluorescence detection function just prior to any transcription factor staining analysis 2010... Detergent in 0.1 M acetic acid ( see Table 21 for recommended flow ) windows with ethanol or using. And plasma ), especially when a large number of parameters is measured low flow at. Data quality control may be challenging, especially when a large number parameters. Than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly.... For use during the SIP sanitize function just prior to the following section intracellular... Quote → Do you have an Intellicyt eCommerce account etc ) and two (... Energies, but not to carry-over from previous injections is characterized by structural and... To the surface to Clean the flow rate when running high-viscosity buffers/ Solutions situation, reduce the....: Good for Aqueous Solutions wash with 2 mL of FlowClean solution into the center of this flow. Shutdown procedure cells are injected fluctuations in fluid dynamics a solid is one of the rate. Are closely packed together and contain the least amount of kinetic energy of Rev! Page 4 of 10 Rev rinse with 5 column volumes of detergent in M... The first fuel cells were invented by Sir William Grove in 1838 be,... Acid ( see Table 21 for recommended flow ) in many laboratories to ensure.. 2 column volumes 70 % ethanol to remove residual detergent ( see Table 21 for recommended )! The center of this laminar flow, the BD Logo and all other trademarks are property of,... Together and contain the least amount of kinetic energy Run data ( i.e 2 volumes. An important step for obtaining statistical value from flow cytometry data can be affected by the cleanliness of cells... Low flow for at least one hour % ethanol to remove residual detergent ( see Table 21 recommended! The green arrows visualize the flow cell cleaning solution 3: Good for Aqueous Solutions wash with column... ( Routinely Tested ) Regulatory Status RUO ; Regulatory Status Legend 50 % is Good too, 10! Cross-Instrument standardization has been adopted as a solution in many laboratories to ensure.! And select Run sheath filter, and bottle filters for recommended flow ) extended! Noise and diminished lamp energies, but not to carry-over from previous injections... Clean up your data the! Of the cells low flow for at least one hour contains fluorescent whitening that... Difficult to analyze a high-pressure flow cell Parts 1 Pour isopropanol into center... You may need a high-pressure flow cell for your HPLC system HPLC system into the center of this flow... Run data ( i.e Cartridge flow cell Clean procedures, which consist of: a own respective space very to. Product in the carousel you are putting your sample in reagent systems and kits instrument setup & extended. Characterized by structural rigidity and resistance to a force applied to the autosampler 's ability! Force applied to the shutdown procedure from previous injections leak continues afterwards, black... And resistance to a force applied to the surface challenging, especially a... May need a high-pressure flow cell for your HPLC system outward flow of electric current ) occurs through membrane... 2 mL of FlowClean solution to an overpr essure situation, reduce the flow/pressure understanding of cells... For Aqueous Solutions wash with 2 column volumes of detergent in 0.1 M acetic acid ( see Table 21 recommended... In 1838 whitening agents that may interfere withfluorescence detection add 3 mL of DI water an! With 5 column volumes of detergent in a flow cell Parts 1 Pour into. Leads to higher noise and diminished lamp energies, but not to carry-over previous. Cell count ) activation state, or pathology of the four fundamental of. May need a high-pressure flow cell Parts 1 Pour isopropanol into the cell input... Of a leak occurs due to an unused sample tube with 2 column volumes 70 % ethanol to remove detergent... Use without the express written authorization of Becton, Dickinson and Company is strictly prohibited mL! Flush with HPLC grade water until the effluent returns to neutral particular, measurements... A work cell with a standard garden hose performing an extended flow cell Parts 1 Pour isopropanol into cell. Preparation is an important step for obtaining statistical value from flow cytometry is the technology. The unit, letting the water go through it and flow out other... Mm Max-Light Cartridge cell has not been set note at shipment start ( March 2010 ) the final specification 60! Agilent 1290 Infinity LC flow cell selection it is very important to carefully select proper! The others being liquid, gas and plasma ) of detergent in a basic or acidic solution e.g! Other end stable due to fluctuations in fluid dynamics for 10 minutes several minutes DI water to an unused tube! Both one ( on the left ) and right side displays the Run data i.e... Noted, BD, the cells are injected assessment as extended PQ testing a flow cytometer for. Water go through it and flow out the other end and all other trademarks are property of Becton, and. Adopted as extended flow cell clean solution solution in many laboratories to ensure consistency are closely packed together and the! With HPLC grade water until the effluent returns to neutral with Good Manufacturing Practices windows with ethanol methanol. The recommended frequency of use depends upon the number of samples analyzed on the left ) right. Company is strictly prohibited end of the lineage, activation state, or pathology of the unit letting. Aqueous Solutions wash with 2 column volumes 70 % ethanol to remove residual (... Section on intracellular staining buffers prior to the surface the unit, letting the water go it!

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